林荫银莲花组培苗生根培养基的优化

Optimization of Rooting Medium for Regenerated Shoots of Anemone flaccida

以药用植物林荫银莲花不定芽组培苗为试验材料,研究培养基添加不同植物激素、不同质量浓度蔗糖和活性炭对组培苗生根的影响。结果表明:以1/2 MS为基本培养基,添加0.5 mg/L NAA+0.5 mg/L IBA,30 g/L蔗糖作为碳源,另加入1.0 g/L活性炭,培养55 d后,林荫银莲花组培苗的生根率为93.9%,平均根条数为10.5,根长最长达1.73 cm,根较粗且褐化轻微。

Effects of plant hormones,sucrose and active carbon contained in the culture medium on rooting of regenerated shoots were studied using regenerated shoots of Anemone flaccida as explants.The results showed that 1/2 MS supplemented with NAA 0.5 mg/L and IBA 0.5 mg/L,30.0 g/L sucrose as carbon sucrose and added with active carbon 1.0 g/L was suitable for rooting of A.flaccida.Under this medium the rate of rooting can reach up to 93.9%,number of root was 10.5 per explant and length of root was 1.73 cm（the longest）.After culturing for 55 d root was more thick and less brown.Rooting system of A.flaccida can be optimized by increasing quantity and quality of regenerated root,thus increasing survival rate of transplanting.