紧密连接蛋白claudin-10基因5′调控区序列的生物信息学分析

Bioinformatics analysis of 5' regulatory region of the tight junction protein claudin-10 gene

目的:研究紧密连接蛋白对claudin-10基因转录调控机制,并对潜在的转录因子结合位点进行分析。方法:利用BLAST比对分析TATA-box、GC-box和CAAT-box;利用在线分析软件Neural NetworkPromoter Prediction、PROMOTERSCAN和PROMOTER2.0分析claudin-10基因5′调控区序列中启动子;利用在线分析软件EMBOSS和CpGIsland Searcher分析claudin-10基因5′调控区序列中CpG岛位置;利用在线分析软件TFSEARCH分析claudin-10基因5′调控区序列中潜在的转录因子结合位点。结果:claudin-10基因5′调控区序列中存在1个CAAT-box和3个GC-box,没有TATA-box;claudin-10基因可能存在4个启动子位点;CpG岛为444bp区间(1700bp-2143bp)或834bp区间(1367bp-2200bp);评分在85分以上时,该区域具有159个潜在的转录因子结合位点;评分在90分以上时,该区域具有48个潜在的转录因子结合位点;评分在95分以上时,该区域具有9个潜在的转录因子结合位点。结论:通过生物信息学的方法对于claudin-10基因转录起始位点、启动子区域和潜在的转录因子结合位点进行预测,对于科研具有十分重要的指导意义,但最终的结论还需要实验来证实。

Objective： To study transcription and regulation mechanisms of the tight junction pro- tein claudin-10 gene, and to analyze its potential transcription factor binding sites. Methods= TATA-box, GC-box and CAAT-box were analyzed with Basic Local Alignment Search Tool （BLAST） ; promoter sites, CpP island and the potential transcription factor binding sites of 5 regulatory region of claudin-10 gene were analyzed with on-line software of Neural Network Promoter Prediction, Promoter Scan, Promoter 2.0, EMBOSS, CpG Island Searcher, and TFSEARCH seperately. Results.. In 5 ＂regulatory region of the claudin-10 gene, a CAAT-box, 3 GC-box, but no TATA-box was found; 4 promoter sites might be pres- ented in claudin-10 gene; CpG island was 444 bp （1 700 bp 2 143 bp） or 834 bp （1 367 bp-2 200 bp） in length. On circumstances of scores of 85, 90, and 95 （or above）, 159, 48 and 9 potential transcription factor binding sites were separately found. Conclusion.- The study of promoter sites, potential transcription factor binding sites for claudin-10 gene with bioinformatics approach is of great significance, but final conclusion needs to be confirmed by further experiments.