血管性血友病因子和胎盘生长因子检测在AMI中的临床意义

Clinical significance of detection of Von Willebrand factor and placental growth factor in patients with acute myocardial infarction

目的:观察急性心肌梗死(Acute Myocardial Infarction,AMI)患者循环内皮细胞(CEC)核DNA改变的特点,检测血浆中血管性血友病因子(Von Willebrand factor,v WF)、胎盘生长因子(Placentalgrowth factor,PLGF)的含量变化,探讨其临床意义。方法:应用单细胞凝胶电泳技术(SCGE)检测102例AMI患者(AMI组)和75例健康人(对照组)CEC核DNA的损伤;以酶联免疫吸附实验法(ELISA)进一步检测两组v WF、PLGF的含量。结果:SCGE显示:AMI组患者(93.72±6.75)%CEC核DNA电泳呈明显的"彗星"状,其彗尾DNA含量为(42.22±1.56)%;对照组(10.24±4.33)%CEC核DNA表现为"彗星"状,其彗尾DNA含量为(6.31±2.41)%。AMI组与对照组彗星率、彗尾DNA含量比较差异均有显著性意义(P均<0.001)。AMI组v WF、PLGF的含量显著高于对照组(P<0.01)。结论:AMI组患者血管内皮细胞明显损伤;血浆中v WF、PLGF的含量明显升高,早期检测血浆v WF、PLGF的含量可能有利于AMI的治疗,甚至可以起到预防作用。

Objective; To observe the alteration of nuclear DNA of circulating endothelial cells, and changes of plasma Von Willebrand factor （vWF） and placental growth factor （PLGF） concentrations in pa- tients with acute myocardial infarction in order to explore its clinical significance. Methods： The damage of nuclear DNA in circulating endothelial cells was assayed by single cell gel electrophoresis （SCGE）; concen- trations of vWF and PLGF were detected by ELISA in 102 patients with acute myocardial infarction （AMI group） and 75 healthy people （control group）. Results： In the AMI group, （93. 72%±6.75） % nuclear DNA in circulating endothelial cells were shown as comet and the DNA content in the tail was （42.22%±1.56） %, significant higher than （10.24±4.33）% and （6.31±2.41） %（ both P〈0.05） of the control group. The concentrations of vWF and PLGF were also both significantly higher than that of the control group （P〈0.01）. Oonclusions： In patients with AMI, the vascular endothelial cells are significantly damaged; the contents of vWF and PLGF were significantly increased, thus early detection of plasma vWF and PLGF levels may contribute to the treatment and prevention of AMI.