固态发酵生产高活力纤维二糖酶

Cellobiase Production by a Selected Aspergillus niger Strain in Solid State Fermentation

在固态发酵条件下，对７个纤维二糖酶生产菌株进行了筛选，发现ＡｓｐｅｒｇｉｌｕｓｎｉｇｅｒＬＯＲＲＥ０１２为纤维二糖酶高产菌株。研究了培养时间、温度、培养基含水量及初始ｐＨ等因子对该菌形成纤维二糖酶的影响。当培养基采用自然ｐＨ（约６．０）、含水量７０％，接种后在３０℃下先培养２ｄ，再在２５℃下培养２ｄ，每克干酶曲所含的纤维二糖酶活力可达到４３０．５６ＩＵ。由此获得的纤维二糖酶曲与Ｔｒｉｃｈｏｄｅｒｍａｒｅｓｅｉ产生的纤维素酶曲在糖化纸浆过程中有明显的协同作用，当两者以１∶１０混合使用时（纤维二糖酶与滤纸酶活力之比约为０．４４），可有效地消除水解产物中纤维二糖的累积，使纤维纸浆的酶水解得率高达９０．７％。

Cellobiase production was studied in solid state fermentation by a selected Aspergillus niger strain. The effects of culture time, temperature, initial pH value and water content of the substrate on cellobiase synthesis were discussed. It was found that the cellobiase activity could reach 430.56 IU/g dry koji under suitable fermentation conditions. The synergistic action between the cellobiase enriched koji produced by A. niger and the cellulase koji by Trichoderma reesei was obvious during the hydrolysis of waste pulp. When the two kinds of koji were mixed and the ratio of cellobiase activity to FPA is about 0.4, no cellobiose accumulated in the hydrolysate, and the high hydrolysis efficiency (90.7%) was obtained.