木低聚糖的分离提纯和定性分析

Isolation and Purification of Xylooligosaccharides

分离提纯是制备功能性木低聚糖的关键技术之一。本文利用聚丙烯酰胺凝胶（Ｂｉｏ－ＧｅｌＰ－４）柱层析技术能将木低聚糖混合样品较好地分离开来。当分离条件为：层析柱尺寸Φ３．０×１２０ｃｍ，去离子水为洗脱液，流速２５ｍｌ／ｈ，柱温５０℃，可以得到木二糖至木四糖的单一组分。研究表明，采用ＡｍｉｎｅｘＨＰＸ－４２Ａ糖柱是测定低聚糖的简便快速的好方法。此外，在无高效液相设备的条件下，通过ＤＮＳ法测定还原糖浓度，也可以初步判断低聚糖的分离效果，不失为一种简单、快速和经济的分析方法。

Xylooligosaccharides with specific biological activities have been paid great attention. This study found that xylooligosaccharides could be isolated effectively on a polyacrylamide (BioGel P4) column and some pure samples such as xylobiose, xylotriose and xylotetraose were obtained. The suitable separating conditions were: column size, 3.0120cm; mobile phase, deionized water; flow rate, 25ml/h; column temperature, 50. In addition, xylooligosaccharides could be determined by HPLC on Aminex HPX42A column excellently.