鼻咽癌^(18)F-FDG PET/CT标准化摄取值与肿瘤组织葡萄糖转运蛋白-1及增殖细胞核抗原过度表达的关系

Relationship between standard uptake value of ^(18)F-FDG PET/CT and overexpression of glucose transporter-1 and proliferating cell nuclear antigen in nasopharyngeal carcinoma

背景与目的:恶性肿瘤的显著特征就是葡萄糖代谢增加,鼻咽癌的葡萄糖代谢增加可能与葡萄糖转运蛋白-1(Glut-1)有关,也可能与鼻咽癌细胞的快速增殖有关。本研究探讨鼻咽癌组织18F-脱氧葡萄糖(18F-FDG)的摄取与肿瘤组织葡萄糖转运蛋白及增殖细胞核抗原表达的关系。方法:对2005年3月至2006年8月间本院收治的40例鼻咽癌患者进行正电子发射体层显像检查,测定肿瘤最大摄取值,应用标准链霉菌抗生物素蛋白-过氧化物酶亲和免疫组化SP法检测40例患者肿瘤组织葡萄糖转运蛋白-1和增殖细胞核抗原的表达。结果:40例鼻咽癌组织的最大标准化摄取值SUVmax为9.45±1.87;40例鼻咽癌组织Glut-1阳性细胞率为45.18%,PCNA染色阳性细胞率为36.18%;鼻咽癌组织18F-FDG摄取(SUVmax)和组织Glut-1表达的阳性程度相关(r=0.369,P=0.019),鼻咽癌组织18F-FDG摄取(SUVmax)和组织PCNA表达的阳性程度无相关(r=0.135,P=0.407)。结论:鼻咽癌组织18F-FDG摄取与Glut-1过度表达相关,与PCNA表达无关。

Background and purpose： Increased glucose metabolism is a characteristic of malignant tumors. This characteristic might be related to a facilitative glucose transporter （Glut-1） and the proliferating activities of tumors. This study was aimed to assess the relationship among the over-expression of facilitative glucose transporter （Glut-1） and proliferating cell nuclear antigen （PCNA） as well as the fluorine-18 fluorodeoxyglucose （^18F-FDG） uptake of tumors in patients with nasopharyngeal carcinoma. Methods： Between March 2005 and August 2006, 40 patients with nasopharyngeal carcinoma were imaged with ^18F-FDG positron emission tomography （PET）.Their maximum standard uptake values （SUVmax） were measured. The expression of Glut-1 and the proliferating cell nuclear antigen （PCNA） in the 40 cases were studied in paraffin sections by SP immunohistochemistry. Results： The ^18F-FDG uptake of tumors of the 40 patients with nasopharyngeal carcinoma was 9.44-1.9 （SUVmax）. All 40 tumors tested Glut-1 positive and PCNA positive. The Glut-1 positive cells consisted of 45.18% of the tumor cell area, whereas the PCNA positive cells consisted of 36.18% of the tumor cell area. There were correlations between Glut-1 expression levels （r=0.369, P=0.019） and the tumors＇ ^18F-FDG uptake but no correlations were found between PCNA expression level and the tumors＇^18F-FDG uptake （r=0.135, P=0.407）. Conclusion： Glut-1 over-expression correlates with ^18F-FDG uptake whereas PCNA over-expression does not correlate with ^18F-FDG uptake in patients with nasopharyngeal carcinoma.