Bcl-2和Fas在人食管癌细胞Ec-9706细胞中表达及中药干预作用

THE GENE EXPRESSION OF BCL-2 AND FAS IN HUMAN ESOPHAGEAL EPITHELIA EC-9706 CELLS AND INTERFERENCE OF CHINESE MEDICINE

目的:研究白毛藤诱导人食管癌细胞凋亡的作用及其机制。方法:荧光显微镜观察不同浓度白毛藤诱导Ec-9706细胞凋亡的作用,RT-PCR检测Bcl-2、Fas基因表达量的变化。结果:白毛藤具有诱导Ec-9706细胞凋亡的作用,并且上调Fas基因、降低Bcl-2基因的表达。结论:白毛藤促进Ec-9706细胞凋亡,其机制可能与激活Fas基因、抑制Bcl-2基因表达有关。

Objective：To study the effect of Solamum lyratum Thunb on apoptosis of Ec-9706 cells and its mechanism.Methods：Ec-9706 cells were treated with various doses of Solamum lyratum Thunb.We observed apoptosis of Ec-9706 cells by fluorescence microscope.We detected the levels of Bcl-2 and Fas gene expression in all groups by RT-PCR.Results： Solamum lyratum Thunb has obviously apoptosis-inducing effects on Ec-9706 cells and could effectively decrease the level of Bcl-2 gene expression and improve the level of Fas gene expression.Conclusion： Solamum lyratum Thunb could induce apoptosis of Ec-9706 cells probably through down regulating Bcl-2 gene expression and up regulating Fas gene expression.