新疆紫草毛状根提取物的抑菌活性研究

Study on antibacterial activity of Arnebia euchroma(Royle) Johnst hairy roots extract

为了研究新疆紫草毛状根的抑菌活性与新疆紫草根的抑菌活性是否相似,以甜瓜叶斑病、大肠杆菌、金黄色葡萄球菌、棉花枯萎病菌和棉花黄萎病菌5种菌为供试菌,采用滤纸片法和菌丝生长速率法,对新疆紫草毛状根紫草素粗提物及多糖进行抑菌活性研究。结果表明:乙醇提取物对3种细菌均具有抑制作用,其中对金黄色葡萄球菌的抑菌作用最强。丙酮提取物仅对甜瓜叶斑病菌有抑制作用,其抑制作用较乙醇提取物高26.5%。乙酸乙酯和石油醚提取物对3种细菌均无抑制作用;4种溶剂提取物对2种真菌均有抑制作用,且对棉花黄萎病菌的抑制率均高于枯萎病菌;乙醇提取物对金黄色葡萄球菌、丙酮提取物对甜瓜叶斑病菌的MIC均为0.1 mg/mL,乙醇提取物对大肠杆菌的MIC为6.25 mg/mL;毛状根多糖对2种真菌均具有抑制作用,且对棉花黄萎病的抑制作用较棉花枯萎病高26.14%,而对3种细菌均无抑制作用。

The Pseudomonas syringae pv. melonis, Escherichia coli, Staphylococcus aureus, Fusariurn oxysporum f. sp. vasinfectum and Verticillium dahliae were chosen in bacteriostasis experiments. By filter paper method and mycelium growth rate method, inhibitive activities of crude extract and polysaccharides from Arnebia euchroma （Royle） Johnst Hairy Roots were experimented. This shows that ethanol extract had significant antibacterial action on three kinds of bacteria, and the best inhibitory effect on S. aureus. Acetone extract had bacteriostatic action on P. syringae pv. Melonis,whose inhibitory effect was greater than the ethanol extract of 26.5 %. Ethyl acetate and petroleum ether extract had no inhibition effects on three species of bacteria. Four kinds of different solvent extracts had inhibition effects on two species of fungi, whose inhibition rate of V. dahliae was higher than F. oxysporurn f. sp. vasinfectum. Ethanol extract for S. aureus and acetone extract for P. syringae pv. melonis the MIC was 0.1 mg/ mL. Ethanol extract the MIC was 6.25 mg/mI, for E. coli. Polysaccharides of hairy roots had inhibition effects on two species of fungi, with inhibition of V. dahliae 26.14% higher than F. oxysporun7 f. sp. vasinJectum, but had no inhibition effects on three species of bacteria.