STR分型技术在马亲子鉴定中的应用

Application of STR Loci Analysis in Parentage Identification of Horse

利用STR分型技术进行马亲子鉴定。取VHL20、HTG4、AHT4、HMS7、HTG6、HMS6、AHT5、ASB2、HTG7、HMS3、HMS2共11个STR(短串联重复序列)基因座,运用PCR技术对争议小马、嫌疑母马以及无关对照马的DNA进行扩增,扩增产物经遗传分析仪检测,用GeneMap-per片段分析软件对检测结果进行分析。结果表明,争议小马与嫌疑母马无亲子关系,STR基因分型技术能够准确、快速地进行马亲子鉴定,所用的STR位点具有较高的鉴别效率。

To apply short tandem repeat （STR） loci in the parentage identification of horse, eleven short tandem repeat （STR） loci including VHL20, HTG4, AHT4, HMS7, HTG6, HMS6, AHTS,ASB2,HTG7, HMS3, and HMS2 on DNA of three horses were amplified by PCR. The PCR products were further separated and analyzed in a genetic analyzer （AB 3130xl）. The results showed that the paternity probability between the young horse and the mare was ruled out. This indicates that most of the STR locus used presently is high performance and STR loci analysis was a validation method for parentage identification in horse.