拳参正丁醇提取物对视网膜缺血再灌注损伤时一氧化氮及一氧化氮合酶的影响

Effect of PBNA on the NO Content and NOS Activity in Ischemia/Reperfusion Injury in the Rat Retina

目的研究拳参正丁醇提取物(PBNA)对大鼠视网膜缺血再灌注损伤时NO及NOS活性的影响。方法 SD大鼠随机分成4组:假手术组、视网膜缺血再灌注模型组、低剂量及高剂量PBNA治疗组。假手术组分离颈总动脉后舌下静脉给予1ml.kg-1的NS,其余各组分别在夹闭颈总动脉前分别舌下静脉注射生理盐水、低剂量(0.3mg·kg-1)及高剂量(1mg·kg-1)的PBNA。颈总动脉夹闭1h再灌注1h后,视网膜电流图(ERG)检查,取血,分离血清,测定血清NO含量、T-NOS、iNOS及eNOS的活性。结果与假手术组相比,模型组ERG幅度明显降低(P<0.01)、血清NO含量降低(P<0.001)、iNOS活性升高(P<0.05)、eNOS活性降低(P<0.05);与模型组比较,PBNA低剂量组NO含量升高(P<0.05)、iNOS活性降低(P<0.05),低剂量组及高剂量组ERG幅度升高(P<0.001)、T-NOS(P<0.01)及eNOS(P<0.05,P<0.01)活性均升高。结论 PBNA可通过提高T-NOS和具有保护作用的eNOS活性,降低具有损伤作用的iNOS的活性,升高NO含量,提高抗氧化能力和扩血管功能,对视网膜功能起保护作用。

Objective To investigate the effect of Polygonum bistorta L.n-butyl alcohol（PBNA）extract on the NO content and NOS activity in ischemia/reperfusion（I/R）injury in the rat retina.Methods The rats were randomly divided into four groups：control group,retinal I/R injury group,low-dosage PBNA treated group and high-dosage treated PBNA group.The control group was injected with 1 ml·kg-1 NS through sublingual vein after CCA was dissociated.Other groups were treated with normal saline or PBNA before occlusion of CCA.After occlusion of CCA for 1 hour following reperfusion for 1 hour,electroretinogram（ERG）was surveyed,and serum was separated to determine the content of NO,the activities of T-NOS,iNOS and eNOS.Results（1）The amplitude of ERG in I/R group showed lower values than that in control group（P〈0.01）and two dosage PBNA treated groups（P〈0.001）.（2）The amplitude of ERG and content of NO in I/R group showed lower values than those in control group（P〈0.001）and low-dosage PBNA treated gr...更多oup（P〈0.05）.（3）The activities of T-NOS in both low-dosage PBNA group and high-dosage group were increased,compared with I/R group（P〈0.01）.（4）The activity of serum iNOS in I/R group was incerased compared with control group（P〈0.05）and low-dosage PBNA treated group evidently（P〈0.05）.（5）The activity of serum eNOS in I/R group was decreased compared with control group（P〈0.05）,both low-dosage（P〈0.05）and high-dosage PBNA（P〈0.01）treated groups markedly.Conclusion PBNA has therapeutic effect on retinal ischemia/reperfusion injury by increasing the activities of T-NOS and eNOS,decreasing the activity of iNOS,elevating the content of NO,enhancing the anti-oxidation and dilating the blood vessel. 还原