超临界CO_2萃取南烛叶有效成分的色谱分析

Chromatography analysis on constituents of leaves of Vaccinium bracteatum Thunb by supercritical CO 2 extraction

用超临界ＣＯ２对南烛叶冻干粉进行萃取，并对萃取物进行气相、高效液相色谱分析。结果表明，南烛叶中含有Ｃ０１６（棕榈酸）、Ｃ０１８（硬脂酸）、Ｃ１１８（油酸）、Ｃ２１８（亚油酸）、Ｃ３１８（α－亚麻酸）和Ｃ０２０（花生酸）等６种脂肪酸，其中Ｃ３１８（α－亚麻酸）含量最高，达３６．４％；南烛叶还含有槲皮素和其它黄酮类化合物，槲皮素在萃取物中占３．５２％，黄酮总量占１１．６４％。

By means of supercritical CO 2 extraction, fatty acids and flavonoid components were extracted from the leaves of Vaccinium bracteatum Thunb. The constituents were analyzed by gas chromatography and high performance liquid chromatography. The experiment results indicated that the fatty acids contain 6 components, that is C 0 16 ,C 0 18 ,C 1 18 ,C 2 18 ,C 3 18 and C 0 20 , and the content of α linolenic acid(C 3 18 )is the highest (up to 36 4%). The total flavonoid components reach to 11 64% and melanin to 3 52%