摘要
卡那霉素对棉花胚性愈伤组织的增殖、胚胎发生及发育均有一定的抑制作用，但不同浓度的卡那霉素对棉花胚性愈伤组织的生长发育作用程度不同，低浓度较弱，高浓度较强；胚性愈伤与体细胞胚生长发育的不同阶段对卡那霉素的敏感性有所不同。在棉花遗传转化过程中，卡那霉素浓度不能低于５０ｍｇ·Ｌ－１，胚性愈伤的继代培养时间应控制在３０ｄ左右。
As an antibiotics in the selection medium, kanamycin inhibited the differentiation and development of cotton somatic embryos. But the effect of concentration of kanamycin on the proliferation of cotton embryogenic callus, the differentiations and development of cotton somatic embryos were various. The sensitive of embryogenic callus and somatic embryos to kanamycin were different during the differentiation and development stage. The optimum kanamycin concentration should be higher than 50mg·L -1 , and the generation interval time of embryogenic callus must be controlled at about 30 days for anti-kanamycin cotton selection medium.
出处
《棉花学报》
CSCD
北大核心
1999年第2期70-72,共3页
Cotton Science
基金
农业部重点科研计划
