吉林省玉米骨干自交系及主推杂交种指纹图谱的构建

Fingerprints of the Main Inbred-lines and Hybrids of Maize in Jilin province

本文以吉林省玉米骨干自交系及主推杂交种近５０份为试材，利用ＲＡＰＤ分子标记技术，经过４０个引物的重复筛选，构建了一套完整的ＤＮＡ指纹图谱，其中仅用４个引物就可以完全区分开所有的供试材料，并与蛋白、同工酶等分子标记进行比较，结果表明，多种分子标记手段相结合，将使玉米种子纯度及品种鉴定更完善。

Fifty main inbred lines and hybrids of maize in Jilin Province were studied in this experiment.Utilizing RAPD(Random Amplified Polymorphism DNA),repetitively screening with 40 primers,a complete DNA fingerprinting was constructed.All of the materials can be discriminated with only 4 primers of them.Compared with seed protein electrophoresis analysis and SOD isozyme analysis,it was concluded that using several molecular marking techniques together can make identification of seeds purty and varieties of maize more easily and completely.