抵当汤及其加味方体外抗肿瘤活性研究

Study on in Vitro Antitumor Activities of Didang Decoction and its Jia Wei Formula

目的探讨抵当汤及分别加入柴胡、桂枝、黄芪3个加味方汤剂对人乳腺癌MCF-7细胞、人结肠癌HCT-28细胞、人肝癌HepG-2细胞的体外抗肿瘤活性的影响。方法用MTT法研究抵当汤及3个加味方制剂对MCF-7细胞、HCT-28细胞、HepG-2细胞的生长抑制作用。结果抵当汤对MCF-7、HepG-2细胞有明显的生长抑制作用(P<0.01),分别加入柴胡、桂枝、黄芪3个加味方汤剂对3种癌细胞生长抑制作用有明显提高(P<0.01);4种汤剂在72 h内对MCF-7、HCT-28细胞的毒性呈时间依赖性,IC50值在加味方后显著降低,加入柴胡、桂枝方IC50值可减少至原方的0.79倍至0.25倍。结论抵当汤对体外肿瘤细胞有活性作用,而加入柴胡、桂枝、黄芪的3个加味方活性作用均大于原方抵当汤活性作用,其中加入柴胡、桂枝的活性作用强于黄芪,抵当汤及加味方对人乳腺癌MCF-7细胞的生长抑制最为明显。

OBJECTIVE the purpose of this study is to probe into the influence of Didang Decoction and its combinations with radix bupleuri,cassiatwig or Astragalus mongholicus on the in Vitro Antitumor Activities of Mammary Cancer MCF-7 cells,Colon Cancer HCT-8 cells and Hepatoma HepG-2 cells.METHOD MTT method was adopted to study the inhibiting effects of Didang Decoction and its three Jia Wei Formulae on Mammary Cancer MCF-7 cells,Colon Cancer HCT-8 cells and Hepatoma HepG-2 cells.RESULT Distinct inhibiting effects of Didang Decoction on Mammary Cancer MCF-7 cells,Colon Cancer HCT-8 cells and Hepatoma HepG-2 cells were detected,and after the above three Jia Wei Formulae were respectively added to Didang Decoction,an remarkably increased inhibiting effect was observed.The four Decoctions,as to the toxicity of the MCF-7、HCT-28 cells,were time-dependent.The IC50 value dropped dramatically after adding the Jia Wei Formula,and it even declined to 0.79 times to 0.25 times of the original.CONCLUSION Didang Decoction has active effect on the in Vitro tumor cells,and this effect is enhanced after adding the Jia Wei Formulae like radix bupleuri,cassia twig or Astragalus mongholicus,and the effect is more obvious in the decoction with radix bupleuri or cassia twig,while the most evident inhibition of Didang Decoction and its Jia Wei Formulae was observed in breast cancer cell MCF-7.