不同刺激参数对SD大鼠肌肉收缩的影响

Effects of different electric stimulation parameters on muscle contraction in SD rats

目的研究不同电刺激参数对SD大鼠肌肉收缩的影响,为临床应用电刺激治疗周围神经损伤提供参考。方法采用低频脉冲发射器对SD大鼠的颈项部肌群进行局部电刺激。刺激时改变频率、脉宽、电压及占空比等参数,观察参数变化对肌肉收缩及动物行为的影响。同时记录受刺激肌肉的肌电图。结果 8只SD大鼠在10~60Hz的频率范围内,随频率增加肌肉收缩率逐步增强。肌电图结果显示在刺激频率不变的前提下,随脉宽的增加（50~200μs）,肌电幅值显著上升,且肌电幅值的变化与大鼠行为改变成正相关。结论不同的刺激参数可影响肌肉的收缩状态与强度,选择合适的参数可使失神经支配肌肉发生有节律地收缩,防止肌萎缩,并有助于建立局部肌痉挛动物模型。

Objective To study the effects of different electric stimulation parameters on muscle contraction of SD rats, and provide reference for treating peripheral nerve injury. Methods The local electric stimulation on neck muscles in SD rats were performed by low frequency pulse transmitter. The electric parameters such as frequency, pulse width, voltage and duty cycle were changed during stimulation, then observed related change in muscle contracture and behavior of the animal, and recorded the electromyogram （ EMG）. Results Muscles in 8 SD rats were stimulated by frequency of 10-60 Hz （10, 20, 30, 40, 50, 60 Hz）, with increasing pulse width （50, 100, 200 μs）, the amplitude of the muscle contracture raise significantly in EMG, and change of amplitude was in positive relation with animal behavior. Conclusion Different electric stimulation parameters can affect the condition and amplitude of muscle contracture. The parameters may be optimized for preventing muscular atrophy and establishing muscle spasm model.