乙基紫测定人血清白蛋白的研究

Research about Ethyl Violet and Human Serum Albumin

研究乙基紫(EV)与人血清白蛋白(HSA)的结合反应.在pH=3.78的条件下,HSA的加入使EV在其最大吸收峰(595 nm)处吸光度增强,比EV溶液的吸光度显著增大.用紫外-可见分光光度计测定,在595 nm处EV-HSA溶液的吸光度值与EV溶液的吸光度值之差ΔA与HSA的浓度c在一定范围内有线性关系,线性响应范围为5.0～150.0 mg/L,检出限为0.097 1 mg/L,用于人血清白蛋白样品测定结果满意,由此建立起快速测定HSA的实用方法.该法具有简便、快速、干扰少、灵敏度高的特点.同时,初步探讨EV-HSA结合机理.

The interaction of ethyl violet（EV） with human serum albumin（HSA） in pH= 3.78 Britton-Robinson buffer solution has been investigated by UV/Vis spectrophotometry technique.The increase in the absorption at 595 nm was proportional to the concentration of HSA base on EV,providing a basis for the quantitative determination of HSA.The absorbance of each solution at 595 nm was measured and the regression equation was ΔA = 0.173 6 ＋ 0.001 5 c（mg/L）.The linear range for the determination of HSA was from 5.0 to 150.0 mg/L with correlation coefficient of 0.999 7 and the detection limit was 0.097 1 mg/L（3σ）.This method was simple and efficient than the ordinary methods and had been applied to the direct determination of HSA with satisfactory results.