Ald^1-Helicokinin Ⅰ的合成研究

Study on the Synthesis of Ald^1-Helicokinin Ⅰ

目的:用荧光非天然氨基酸Aladan(Ald)改造昆虫神经肽Helicokinin Ⅰ。方法:采用多肽固相合成法合成Ald1-Heli-cokinin Ⅰ,基质辅助激光解吸电离质谱确证,经反相高效液相色谱法纯化,纯度达97%以上。结果与结论:Ald1-Helicokinin Ⅰ的合成具有快捷、简便、高效等特点,最终收率31.8%,且Ald1-Helicokinin Ⅰ可作为荧光探针,进一步研究配体跟受体的相互作用。

Objective： To modify the insect neuropeptide Helicolinin Ⅰ by fluorescent non-natural amino acid（Aladan,Ala）.Methods： Ald1-Helicokinin Ⅰ could be synthesized by solid-phase peptide synthesis,confirmed by matrix-assisted laser desorptionionization mass spectrometry,purified by revered-phase high performance liquid chromatography and the purity was up to 97%.Results and discussion： The synthesis method of Ald1-Helicokinin Ⅰ is very fast,simple and high-efficient,the final product rate is 31.8%.Ald1-Helicokinin Ⅰ can be used as fluorescence probe for the further investigation on the interaction between ligand and receptor.