摘要
目的研究间充质干细胞的体内免疫调节活性。方法将C57/BL脾脏细胞输注入受亚致死量照射的BALB/c小鼠,建立急性移植物抗宿主病模型,受体小鼠或同时接受密质骨骨髓来源的间充质干细胞,流式细胞技术检测脾细胞表面表达的活性分子。结果模型经输注间充质干细胞后,鼠脾脏CD11b+细胞表达的CD86和CD69减少,提示抗原呈递细胞的成熟度下降。此外,作为一种效应T细胞早期活化和发育的标记,CD3+CD69+细胞/CD3+细胞的比值降低,导致脾内CD3+细胞的绝对和相对数目减少。但是,在同系脾细胞输注模型中,CD11b+细胞表面的CD69和MHCⅡ表达以及CD3+细胞表面的CD69不受影响。结论 MSCs可以分3个阶段抑制急性移植物抗宿主病,有望成为急性移植物抗宿主病的有效治疗手段,但仍需进行深入研究。
Objective To investigate the in vivo immunoregulatory activities of mesenchymal stem cells (MSCs). Methods A murine aGvHD model was developed by transfusion of C57/BL splenocytes, with or without compact bone-derived MSCs, into sublethally irradiated BALB/c mice. The phenotypic features of the splenocytes were analyzed by flow cytometry after transplantation. Results MSCs infusion decreased the expression of CD86 and CD69 molecules on splenic CD11b+ cells of aGvHD mice, suggesting of the decreased maturation of antigen presenting cells. Meanwhile, the ratio of CD3+ CD69+ to CD3+ cells, a parameter for early activation and effector T cell formation, was dramatically down-regulated, resulting in the enhancement of the absolute and relative number of splenic CD3+ cells by MSCs co-transfer. However, the expression level of MHC Ⅱ and CD69 on CD11b+ cells and CD69 on CD3+ cells were not affected in the syno-splenocyte infusion model. Conclusion The results here suggest that MSCs can inhibit aGvHD at the three development stages of specific cytotoxic T lymphocytes. Further investigations are needed for designing better strategies to manipulate aGvHD in clinical settings.
出处
《组织工程与重建外科杂志》
2010年第3期121-127,共7页
Journal of Tissue Engineering and Reconstructive Surgery
基金
supported by the National Key Basic Research Program of China (Grant number 2005CB522705)
National Natural Sciences foundation of China (Grant number30900568, 30600309, 30730043)~~