大孔树脂纯化柿叶总黄酮的工艺研究

Purification of Flavonoids from Persimmon Leaves by Macroporous Resin

以黄酮的吸附率与解吸率为指标,考察10个不同型号的大孔树脂对柿叶黄酮的吸附与解吸能力,确定NKA-2为较理想的树脂。对NKA-2树脂的纯化条件进行优化,得到最佳工艺条件为:以4 mg/mL的浓度(溶液pH=3)、2 BV/h的速率5 BV上样,以6 BV的70%乙醇、2 BV/h的速率洗脱。经NKA-2处理后的柿叶总黄酮可达52%,纯化了33.8%。

The absorption rate and desorption rate of flavone were taken as indexes to determine the absorption and desorption ability of 10 types of macroporous resin on flavone from persimmon leaves.The result shows that the NKA-2 macroporous resin has good absorbing and desorbing effect.The purification conditions of NKA-2 macroporous resin were optimized,and the optimum conditions were as follows： the liquid concentration was 4 mg/mL（pH=3）,the injection rate was 2 BV/h,the sample volume was 5 BV;eluted with 70% of alcohol of 6BV at the speed of 2 BV/h.After purified by NKA-2 macroporous resin,the flavonoids from persimmon leaves is up to 52%,which is purified by 33.8%.