一株他克莫司产生菌的筛选及鉴定

Isolation and Identification of a Tacrolimus Producing Strain

添加75μg/mLK2Cr2O7和2μg/mL青霉素对土样进行预处理,采用抑菌圈法初筛得到71株能抑制白色假丝酵母生长的菌株,高效液相色谱法检测代谢产物复筛,获得一株他克莫司产生菌株。对菌株形态学特征、培养特征、生理生化特征、细胞化学组分和16SrDNA序列进行分析。结果表明:NJYH2618为革兰氏阳性菌,生长温度15～38℃,最适生长温度28℃,生长pH6.5～8.5,最适pH7.0,能利用葡萄糖、蔗糖、麦芽糖、木糖、果糖、乳糖、鼠李糖等常见碳源。细胞水解产物成分和16SrDNA序列分析表明,此菌株属链霉菌属,命名为Streptomyces sp.NJYH2618。

In order to isolate a tacrolimus producing strain,soil samples were initially treated with 75 μg/mL K2Cr2O7 and 2 μg/mL benzylpenicillin.Seventy-one actinomycetes were isolated by anti-fungal activity against Candida albicans then the metabolites origin from those isolations were further analysis by HPLC.Among these,only one tacrolimus producing strain was determined.It was identified by culture characteristics,morphological characteristics,physiological,biochemical properties and 16S rDNA sequence and phylogenetic analysis.The screened strain was gram-positive,the growth temperature ranged from 15 ℃ to 38 ℃（the optimum was 28 ℃）;the growth pH ranged from 6.5 to 8.5（the optimum at 7.0）.The strain can grown on glucose,sucrose,maltose,xylose,fructose,lactose and rhamnose.Based on the results of the general taxonomical characteristics and the 16S rDNA sequence,the isolated has proven to be Streptomyces and named Streptomyces sp.NJYH2618.