包埋吸附法固定化酒用酸性脲酶的研究

Immobilization of Acid Urease Via Adsorption and Embedded

用包埋吸附法固定化酒用酸性脲酶,探讨了固定化的条件、固定化酶的部分性质以及固定化酶在黄酒中的应用。结果表明:固定化酒用酸性脲酶的最适海藻酸钠浓度、CaCl2质量浓度和壳聚糖浓度分别为2.0、5.0和0.3g/dL,最适固定化时间为2.5h,固定化率为82.3%;最适反应温度40℃、最适pH4.0、半衰期为48d,固定化酶的比活为3.6U/mg;在35℃下添加量为0.1g/mL(固定化酶质量/黄酒体积),24h内黄酒尿素去除率达65%,同样条件下连续使用去除黄酒中尿素,使用13次时尿素去除率达39.7%。

The immobilization conditions,and application acid urease via adsorption and embedded were investigated in this study.The optimum concentration of sodium alginate,calcium chloride and chitosan was 2.0,5.0 and 3.0 g/dL,respectively.the optimal immobilized time was 2.5h,the immobilization rate was 82.3%,and the reaction take place at 40℃and pH4.The half-lives of immobilized acid urease was 48 days,the unit of immobilized urease was 3.6U/mg,the urea removal rate was 65% at 35℃,24 h and the addition level of immobilized acid urease was 0.1g/mL （the quality of immobilized enzyme /the volume of Chinese rice wine）,under the same conditions,the urea removal rate still reached at 39.7% under the continuous using thirteen times.