纳他霉素高产菌株发酵罐发酵条件研究

Study of Natamycin High-Yield Strains Fermenter Fermentation Condition

考察溶氧量、补糖及pH值等因素对纳他霉素高产菌株发酵罐生产纳他霉素的影响。以摇瓶发酵结果为依据,确定了发酵罐发酵的最适条件为:控制溶氧在30%～35%,补糖控制在发酵液糖质量浓度约为2g/dL,在纳他霉素大量合成阶段,维持发酵液中的pH值在5.2～5.4。该发酵条件下纳他霉素产量从分批发酵的3.01g/L提高到5.32g/L。

The effect of the nutrition and environmental conditions,including dissolved oxygen,pH,added sugar and other factors on Natamycin production by Streptomyce natalensis SY-4795 were careful investigated,and the optimum conditions listed as follows：the dissolved oxygen 30%～35%,the constant sugar concentration in fermentation broth keep at 2%,pH 5.2～5.4.With the optimum fermentation conditions,natamycin production increase 3.01 g/L to 5.32 g/L in batch fermentation.