响应面法优化灰绿青霉Penicillium glaucum NS16产酶条件

Optimization of Fermentation Condition for Cellulase-Producing Penicillium glaucum NS 16 by Response Surface Methodology

采用响应面法对灰绿青霉Penicillium glaucum NS16生产纤维素酶的发酵条件进行了优化,通过Plackett-Burman实验方法研究有关碳源、氮源、无机盐、发酵时间、摇床转速等18个发酵因子对菌株发酵产纤维素酶活力的影响。结果显示,影响产酶的显著因子是麸皮、CMC的含量和发酵时间。根据实验结果和经验方法对显著影响因子的取值范围进行预估,并采用Box-Behnken设计实验进行优化,然后应用响应面模拟预测和摇瓶发酵实验验证,结果表明,优化发酵条件为培养基中麸皮6.0g/L,CMC7.0g/L,发酵时间96h,摇瓶发酵液中CMC酶活均接近309IU/mL,优化预测值和实验验证值拟合度达到99%,比初始培养基和发酵条件下菌株产CMC酶活94.51IU/mL提高了227%。

In this manuscript,response surface design was used to optimize the nutritional and environmental conditions of cellulase production by Penicillium glaucum NS16.There are 18 factors affect the cellulose production,including the fermentation time,shaking rate,concentration of carbon sources,nitrogen sources and inorganic salts.The influence of the 18 factors was evaluated by Plackett-Burman design.The result showed that wheat bran,CMC-Na and the fermentation time play important roles on the cellulase production.According to the result of Plackett-Burman design experiment,the value range of the significant influential factors could be estimated,and a Box-Behnken design was employed to optimize these factors and the results were shown in response surface graph.Following the results,the optimum conditions listed as follows：6.0 g/L wheat bran,7.0 g/L CMC-Na,and 96 hours feimentation time.By the optimum conditions,309 IU/ml of cellulase was produced by Penicillium glaucum NS16,which was higher 227% than that of the control （94.51 IU/mL）