一株具有拮抗作用的海洋放线菌筛选及其特性

Characteristic of Marine Actinomycete Having Antagonistic Effects

为了探寻对大肠杆菌、金黄色葡萄球菌具有拮抗作用效果的海洋微生物新菌种,作者采用平板稀释方法,经初筛与复筛从海洋淤泥中分离得到一株对大肠杆菌和金黄色葡萄球菌具有较好拮抗作用效果的海洋微生物菌L菌株,并对该菌的生理生化特性、发酵过程参数的变化及抑菌效果进行了研究。结果表明:海洋微生物菌L菌经初步判断属放线菌,L菌适宜的发酵时间为9～10d,产生的发酵产物对大肠杆菌和金黄色葡萄球菌均有一定的拮抗作用。

The purpose of this manuscript is to screen the marine microorganisms and evaluate the antagonistic effects of those isolated microorganism to Escherichia coli and the Staphylococcus.A isolated marine microorganism L having better antagonism effects was separated from the diluted marine mud.Physiology and biochemical characteristics of the isolated marine microorganism,the curve of fermentation control parameters were studied.Antagonism effects of the ferment production were tested with the Escherichia coli and the Staphylococcus.The result shows that marine microorganism L was marine actinomycetes,the suitable fermentation time is 9 to10 days,the secondary metabolite in fermentation broth have certainty the antagonism ability to Escherichia coli and Staphylococcus.