rSj26-Sj32-Immunogold-IgG-Dot-ELISA用于急性日本血吸虫病的诊断价值

Diagnostic Value of rSj26-Sj32-Immunogold-IgG-Dot-ELISA Assay in the Diagnosis of Acute Schistosomiasis Japonicum

目的探讨rSj26-Sj32-Immunogold-IgG-Dot-ELISA用于急性日本血吸虫病患者的诊断价值。方法利用纯化的rSj26-Sj32融合蛋白和日本血吸虫成虫抗原(SjAWA)建立Immunogold-IgG-Dot-ELISA方法检测急性日本血吸虫病患者血清,并以华支睾吸虫病、卫氏并殖吸虫病、棘球蚴病、乙型肝炎、肺结核病患者和健康人血清作对照,比较检测结果。结果 rSj26-Sj32和SjAWA检测急性日本血吸虫病患者的阳性检出率均为100%,特异性分别为97.67%和95.35%;SjAWA与华支睾吸虫病和卫氏并殖吸虫病血清均存在不同程度的交叉反应。结论纯化的rSj26-Sj32融合蛋白是一种较好的免疫诊断抗原。

Objective To study the diagnostic value of rSj26-Sj32 fusion protein-Immunogold-IgG-Dot-ELISA in the diagnosis of acute schistosomiasis japonicum.Methods Purified rSj26-Sj32 protein and Schistosoma japonicum adult worm antigen（SjAWA） were used in the Immunogold-IgG-Dot-ELISA.Sera from patients with acute schistosomiasis japonicum were used in this analysis.Control sera were obtained from patients with paragonimiasis,clonorchiasis,echinococciasis,hepatitis B,lung tuberculosis and the normal subjects.Results All patients with acute schistosomiasis japonicum were positively diagnosed using rSj26-Sj32 fusion protein and SjAWA antigens.The specificity for the testing using rSj26-Sj32 fusion protein and SjAWA was 97.67% and 95.35%,respectively.Cross-reaction was observed in the sera from patients with paragonimiasis and clonorchiasis when SjAWA was used.Conclusion Recombinant Sj26-Sj32 may be used for the diagnosis of schistosomiasis.