Alcaligenes sp. CGMCC2428产威兰胶的分批发酵动力学模型

Kinetics modeling for welan gum production by Alcaligenes sp. CGMCC2428 in batch fermentation

利用分批发酵研究Alcaligenes sp. CGMCC2428产微生物多糖威兰胶的合成特性,结果表明威兰胶的合成和菌体生长模型为部分偶联型.菌体和威兰胶质量浓度分别达到3.30和26.50 g/L,威兰胶对细胞干质量得率系数(YP/X)为8.20.根据分批发酵实验结果采用Logistic方程、Luedeking-Piret方程和Luedeking-Piret相似方程,得到了描述Alcaligenes sp. CGMCC2428生长、威兰胶以及葡萄糖底物消耗分批发酵动力学模型.同时改变初始葡萄糖质量浓度,实验数据与模型预测值进行了比较拟合,平均相对误差小于10%,表现出很好的适用性.

The synthetic of extracellular polysaccharide welan gum of Alcaligenes sp.CGMCC2428 performed in batch fermentation was studied.The result showed that the fermentation mode of Alcaligenes sp.CGMCC2428 was the partial growth-associated type.The dry cell weight（DCW） and welan gum reached 3.30 g/L,and 26.50 g/L.The yield（YP/X） of welan gum to DCW was 8.20.Based on the results of the batch fermentation,a kinetic model was proposed by using the Logistic equation for cell growth,the Luedeking-Piret equation for welan gum production,and the Luedeking-Piret like equation for consumption of glucose as substrate.Compared with experimental data on various initial glucose concentrations,the average relative error of the model was no more than 10%.