SFP-AQ法预处理麦秸秆酶解五碳糖得率的变化规律

EVOLUTION OF ENZYMOLYSIS PENTAGLUCOSE YIELD OF WHEAT STRAW BY SFP-AQ PRETREATMENT

对SFP-AQ法(亚硫酸钠和甲醛-蒽醌)预处理麦秸秆酶解五碳糖(阿拉伯糖和木糖,主要为木糖)的得率进行了研究.结果表明:木糖得率随着预处理中Na2SO3用量的增加先升高后降低,在其质量分数为12%时木糖得率最高;木糖得率随着酶用量的增加而迅速升高,但当酶用量超过20FPU/g时,提高缓慢;木糖得率随蒸煮最高温度的升高和保温时间的延长而增加.较适宜的预处理和酶解条件分别为:蒸煮最高温度160℃,保温时间2h,Na2SO3用量为12%,纤维素酶、木聚糖酶、β-纤维二糖酶3种复合酶用量为20FPU/g.此时,酶解木糖得率可达到17.5%,酶解木糖对麦草原料中木糖的转化率为84.9%.

The yield of the wheat straw which was preprocessed by SFP-AQ（Na2SO3、HCHO-AQ） on enzymolysis pentaglucose（arabinose and xylose, mainly of xylose） was studied in this paper. The result shows that： the yield of arabinose is very low; the yield of xylose increase with the increase of Na2SO3 dosage in the pretreatment at first, then decreased, when the dosage of Na2SO3 is 12% the yield reach the maximum; the yield of xylose increase rapidly with the increase of the dosage of enzymes, when enzymes dosage exceed 20 FPU/g yield increase slowly; the yield of xylose increase rapidly with the increase of the maximum temperature and holding time. The suitable pretreatment and enzymolysis conditions are as follows： cooking temperature 160 ℃; holding time lasts 2 h with 12% of Na2SO3 dosage; the dosage of complex enzymes is 20 FPU/g at 50 ℃ which are composited of cellulase, xylanase, β-portuguese gather glycosidase enzymes. In addition, the enzymolysis process lasts 48 h, enzymolysis xylose yield can reach up to 17.5%, percent conversion of enzymolysis xylose on xylose in wheat straw is 84.9%.