杜仲叶黄酮苷抗疲劳和抗氧化活性的研究

STUDY ON ANTI-FATIGUE AND ANTI-OXIDATION ACTIVITY OF FLAVONOID GLYCOSIDE FROM EUCOMMIA ULMOIDS LEAVES

以昆明小鼠为实验对象,研究了杜仲叶黄酮苷的抗疲劳作用及其与抗氧化活性的关系,探讨了杜仲叶黄酮苷的抗疲劳作用机制.分别对正常对照组和杜仲叶黄酮苷组小鼠进行负重游泳实验,并检测运动后小鼠的肌糖原、肝糖原、全血乳酸(LD)、血尿素氮(BUN)、乳酸脱氢酶(LDH)、谷胱甘肽过氧化物酶(GSH-PX)、超氧化物歧化酶(SOD)和丙二醛(MDA)各项生化指标.结果显示,杜仲叶黄酮苷能显著延长小鼠负重游泳时间,降低血乳酸和血清尿素氮含量,提高肝糖原的含量,降低MDA的含量,提高T-SOD的活性,说明杜仲叶黄酮苷的抗疲劳作用与增加能量储备、减少过度运动后不良代谢物的生成、提高组织的耐受力以及清除运动中产生的大量自由基等作用有关.

The efficacy of flavonold glycoside from Eucommia ulmoids leaves was studied through animal experiments. In addition, the relationship between anti-fatigue effect and antioxidant activity of Eucommia ulmoides leaves is tried to be explored.Kunming mice as the subject animals were divided into two groups as blank control group and flavondod glycoside group. The results of anti-fatigue research indicated flavonold glycoside from Eucommia ulmoids leaves could prolong the weight swimming time,decrease the content of lactic acid and serum urea nitrogen,increase the content of liver glycogen. The results of anti-oxidation in vivo research indicated that flavonold glycoside from Eucommia ulmoids leaves could reduce the content of malonaldehyde（MDA） and increase the activity of superoxide dismutase（SOD） in mice. The active ingredient was,of wich the anti-fatigue effect was related to increasing energy storage,lessening generation of bad metabolites during exercise,improving the body＇s tolerance ability to exercise and clearing the free radical during exercise.