期刊文献+

马铃薯病毒分离提纯的方法

Separation and Purification Methods of Potato Virus
下载PDF
导出
摘要 马铃薯已被我国定为第四大粮食作物,国内马铃薯需求量也越来越大,种植面积逐年上升,同时国际种薯市场也在逐渐扩大,需要大量高质量的合格种薯,所以迫切需要用于检测马铃薯病毒的病毒检测试剂盒。本文主要介绍马铃薯病毒提纯的环节及注意事项,以便提纯出高质量的病毒,用于制备病毒检测试剂盒检测病毒。 Potato is considered as the fourth main food crop in China,which is demanded more and more every year.Thus,the cultivation area is also expanding accordingly with the growing international market and great demand for qualified seed potatoes.Therefore,the testing kit for potato virus diseases is also badly needed.The procedures and notices that guarantee high quality virus in potato virus purification were introduced in this research,so as to prepare the high quality virus detection kit.
出处 《中国马铃薯》 2010年第3期179-182,共4页 Chinese Potato Journal
基金 农业科技成果转化资金项目(2008GB2B200084) 马铃薯产业体系(NYCYTX-15) 948项目(2008-Z23)
关键词 马铃薯 病毒 提纯 potato virus purification
  • 相关文献

参考文献15

  • 1E.库尔斯塔克.植物病毒比较诊断指南[M].北京:农业出版社.1991:518.
  • 2Salazar L F. Harrison B D.. Host range, purification and properties of potato virus[J]. Ann Appl Biol, 1978, 89: 223-224.
  • 3白艳菊,李学湛,于德才,范国权,高艳玲,马纪,于虹,田忠.黑龙江省马铃薯产业发展现状[J].中国马铃薯,2006,20(2):124-127. 被引量:6
  • 4李之芳.中国马铃薯主要病毒图鉴[M].北京:中国农业出版社,2004:4-5.
  • 5田波 裴美云.植物病毒研究方法[M].北京:科学出版社,1987..
  • 6白艳菊,李学湛,吕典秋,何云霞,张儒喜,朱财.应用DAS-ELISA法同时检测多种马铃薯病毒[J].中国马铃薯,2000,14(3):143-144. 被引量:27
  • 7Pierpont W S. The enzymic oxidation of chlorogenic acid and some reactions of the quinine produced[J]. Biochem, 1966, 98: 567-580.
  • 8LUIS FSALAZAR.马铃薯病毒及其防治[M].北京:中国农业科技出版社,2000:6-7,167.
  • 9Makoto K, Eishiro S, Masayoshi S, et al. Purification and electron microscopy of potato leafroll virus{J]. Virology, 1969, 2: 162-174.
  • 10郭兴启,吕士恩,朱汉城,等.烟草上马铃薯Y病毒的提纯与检测[M].北京:中国农业科学技术出版社.1999:5-7.

二级参考文献12

  • 1刘卫平.快速ELISA法鉴定马铃薯病毒[J].马铃薯杂志,1997(1):11-13. 被引量:10
  • 2H. Barker,K. D. Webster,B. Reavy.Detection of potato virus Y in potato tubers: a comparison of polymerase chain reaction and enzyme-linked immunosorbent assay[J].Potato Research.1993(1)
  • 3L. Beczner,J. Horváth,I. Romhányi,H. F?rster.Studies on the etiology of tuber necrotic ringspot disease in potato[J].Potato Research.1984(3)
  • 4Piche L M,Singh R P,Nie X,et al.Diversity among potato virusY isolates obtained from potatoes grown in the United States[].Journal of Virology.2004
  • 5Nie X,Singh R P.Specific differentiation of recombinant PVYN:Oand PVYNTN isolates by multiplex RT-PCR[].Journal of Viro-logical Methods.2003
  • 6Nie X,,Singh,R P.Evolution of North American PVYNTN strainTu 660 from local PVYN by mutation rather than recombination[].Journal of Virology.2003
  • 7NIE X,SINGH R P.A new approach for the simultaneous differentiation of biological and geographical strains of Potato virus Y by uniplex and multiplex RT-PCR[].Journal of Virology.2002
  • 8Matthews R E F.Plant Virology[]..1981
  • 9P. Ellis,R. Stace-Smith,G. de.Villers.Identification and Geographic Doistribution of Serotypes of Potato Virus Y[].Plant Disease.1997
  • 10Barker,H,Webster,KD,Reavy,B.Detection of potato virus Y in potato tubers: a comparison of polymerase chain reaction and enzyme-linked immunosorbent assay[].Potato Research.1993

共引文献63

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部