植物生长调节剂对五鹤续断组织培养的影响

Effect of Plant Growth Regulator on Tissue Culture of Wu-He Dipsacus asper

通过不同植物生长调节剂的组合和配比,对五鹤续断茎尖、幼嫩茎段和叶进行愈伤组织的诱导、丛生芽分化和增值及生根培养,确定了五鹤续断组织培养的最佳培养基为:(1)初代培养基为MS+6-BA1.5 mg/L+NAA0.1mg/L;(2)丛生芽培养基为MS+6-BA 2.0mg/L+NAA 0.6 mg/L;(3)生根培养基为1/2MS+IBA 0.4 mg/L+PP3330.4mg/L.

Shoot tip and tender stems which sprouted at the beginning of spring were used as explants to study effect of plant growth regulator on tissue culture of Wu-He Dipsacus asper.The optimal media were（1）callus medium,MS＋6-BA 1.5 mg/L＋NAA 0.2 mg/L;（2）clump bud induction medium,MS＋6-BA 2.0（mg/L）＋NAA 0.5（mg/L）;（3）rooting medium,1/2MS＋NAA（1.0 mg/L）＋ PP333（0.4 mg/L）.