摘要
目的:用高效液相色谱法同时测定生脉饮中人参皂苷Rb1、Rg1的含量。方法:采用DiamonsilC18柱(5μm,250mm×4.6mm),乙腈-水进行梯度洗脱,流速0.6mL·min-1,检测波长203nm,柱温25℃。结果:对生脉饮中人参皂苷Rg1和人参皂苷Rb1进行含量测定。其线性范围分别为0.050～1.000mg·mL-1(r=0.9994)和0.050～1.000mg·mL-1(r=0.9990),平均回收率分别为96.2%(RSD=2.4%)和98.5%(RSD=2.7%)。结论:方法简便,可用于生脉饮质量的评价。
Objective：To,determine the content of ginsenoside Rb1 and ginsenoside Rg1 in Shengmaiyin simultaneously by HPLC. Method：The quantitative determination was carried out on a Diamonsil C 18 column （5μm,250mm×4.6mm）, with a mobile phase of acetonitrile-water at a flow rate of 0. 6 mL ·mL^-1, and UV detection was performed at 203 nm with 25℃as the column temperature. Result：Quantitative determination of ginsenoside Rb1 and ginsenoside Rg1 was performed. The linear ranges for ginsenoside Rg1 and ginsenoside Rb1 were 0. 050 - 1. 000 mg·mL^-1 （ r = 0. 9994） and 0. 050 - 1. 000 mg·mL^-1 （ r = 0. 9990）, respectively. The average recoveries were 96. 2% （RSD = 2.4% ） and 98.5% （RSD = 2. 7% ）, respectively. Conclusion：The method is simple, and it can be used to evaluate the quality of shengmaiyin.
出处
《中药与临床》
2010年第1期28-30,共3页
Pharmacy and Clinics of Chinese Materia Medica
基金
四川省教育厅科研基金资助项目(No.2006A44)
