光身稻的培养特性研究

Studies of Culture Character in Glabrous Rice(Oryza sativa L. )

本文对光身稻培养特性进行了系统研究。结果指出:(1)光身稻的成熟胚培养力极低,其绿苗分化率几乎等于零,为0.7％,但其幼穗培养力则较高,绿苗分化率为44.8％;(2)光身稻的花药愈伤组织出现时间较早,为25天左右,而且愈伤组织诱导率也较高,为7％,但其绿苗分化率很低,仅为0.35％;(3)光身稻的适宜诱导培养基为M_8;(4)花药愈伤组织的干燥预处理能明显提高其绿苗分化率;(5)光身稻后代二倍体的比例较低,而光身稻出现的比例则较高。

The current paper carried out series of studies on the culture characters of glabrous rice. The experiment results indicated:(1) The culture ability of mature embryo from glabrous rice was very low. The green plantlet redifferentation frequency was almost zero, about 0. 74% , but the culture ability of young panicle was higher its green plantlet redifferentation frequency was 44. 8%; (2)The day that the callus could be seen from anther of glabrous rice was early about 25 days, and the callus in duction frequency was also high, about 7. 4% but its green plantlet redifferentation frequency was very low; (3) The most suitable induction medium for glabrous rice is M8; (4)The dehydration treatment of anther callus can enhance the frequency of green plantlet redifferentation obviously; (6) The rate of deploid for glabrous rice progency was very low, and the rate of glabrous rice was higher.