抑制素被动免疫对大鼠排卵率及血浆雌二醇与孕酮的影响

THE EFFECT OF PASSIVE IMMUNIZATION AGAINST INHIBIN ON OVULATION RATE AND PLASMA ESTRADIOL AND PROGESTERONE IN RATS

本试验研究了注射人抑制素α亚基（１－２６）Ｔｙｒ·Ｇｌｙ抗血清对大鼠排卵率、血浆Ｅ２与Ｐ水平的影响。试验组Ⅰ（ｎ＝５）在动情后期上午１１∶００，试验组Ⅱ（ｎ＝５）间情期上午１１∶００尾静脉注射抗血清，而相应的对照组Ⅰ、对照组Ⅱ在对应时间注射正常绵羊血清。结果显示，在动情后期注射抗血清，试验组大鼠排卵率为１１．２０±０．３２，对照组１０．５５±０．１９，差异不显著，而在间情期注射抗血清，试验组、对照组排卵率分别为１３．８０±０．５５、１０．８０±０．２２，差异显著（Ｐ＜０．０５），试验组比对照组提高２７．８％，说明抑制素被动免疫大鼠可提高排卵率，且在间情期处理效果更好。间情期注射抗血清８ｈ后，试验组、对照组血浆Ｅ２水平分别为４０．４９±３．９６、３９．３６±１．９９ｐｇ／ｍｌ，差异不显著，２１ｈ后试验组、对照组Ｅ２水平分别为３０．０４±１．６７、３０．０６±１．４３ｐｇ／ｍｌ，差异也不显著，说明免疫中和大鼠体内抑制素未能提高血浆Ｅ２水平。间情期注射抗血清８ｈ后，试验组、对照组血浆Ｐ水平分别为５２．１７±３．７２、１７．７９±０．７８ｎｇ／ｍｌ，差异极显著（Ｐ＜０．０１），２１ｈ后试验组、对照组Ｐ水平分别为１９?

Ovulation rate,plasma estradiol(E 2)and progesterone (P)concentrations in rats were studied after a single injection of antiserum against a synthetic peptide of human inhibin α subunit Rats were injected(0 5ml)in cauda vein at 11∶00 on metoestrus(M),or at 11∶00 on dioertrus(D) Control rats received sheep serum(non immune)at 11∶00 on M,or at 11∶00 on D The ovulation rate of rats injected on M was not significantly different from that of control rats(11 20±0 32 vs 10 55±0 19) The ovulation rate was significantly higher in rats injected on D than in control rats(13 80±0 55 vs 10 80±0 22,P<0 05) These results indicated that ovulation rate of rats can be increased by immunoneutralization of inhibin,and more ovulation is induced by injection of antiserum on dioestrus Estradiol level was not significantly different between control rats and rats injected on D 8h,or 21h later after injection (39 36±1 99 vs 40 49±3 96pg/ml,30 06±1 43 vs 30 04±1 67pg/ml).Progesterone level was significantly higher in rats injected on D than in control rats 8h later after injection (52 17±3 72 vs 17 79±0 78ng/ml,P<0 01),but not significantly different 21h later after injection (19 23±1 63 vs 14 55±1 61ng/ml) Therefore,passive immunization against inhibin can not increase estradiol level,but can cause a short time increase of progesterone level in rats