拟除虫菊酯对家蝇Na-K-ATPase抑制作用的研究

INHIBITION OF PYRETHROID INSECTICIDES ON NERVE NA K ATPASE IN HOUSE FLIES (MUSCA DOMESTICA)

通过对家蝇神经系统ＮａＫＡＴＰａｓｅ性质的研究，表明ＮａＫＡＴＰａｓｅ反应的适宜ｐＨ值为７０～８０，适温为３５～４０℃，Ｋｍ为０２２ｍｍｏｌ／Ｌ，Ｖｍａｘ为５５５５６ｎｍｏｌ／（ｍｇ·ｍｉｎ）。比较测定了家蝇敏感品系、ＤｅｌＲ、２ＣｌＲ抗性品系的ＮａＫＡＴＰａｓｅ活性及溴氰菊酯和氯菊酯对该酶的抑制作用。实验证明，敏感与抗性品系间ＮａＫＡＴＰａｓｅ活力无显著的差异，但溴氰菊酯和氯菊酯对不同家蝇品系ＮａＫＡＴＰａｓｅ的抑制作用有明显区别，两种拟除虫菊酯可抑制敏感家蝇品系ＮａＫＡＴＰａｓｅ的活性，而对抗性品系无明显的抑制作用。

The properties of Na K ATPase in the nerve system of house flies were described For Na K ATPase the optimum pH and temperature were 7 0～8 0 and 35～40℃ respectively, the K m value was 0 22 mmol/L ATP and V max was approximately 555 56 nmol Pi/(mg·protein·min). The Na K ATPase activities in susceptible(SP) and Del R、2Cl R house fly strains were determined The results indicated that there was no significant difference in Na K ATPase activities among the three strains Inhibition of deltamethrin and permethrin on Na K ATPase activity was also studied The results showed that the two pyrethroids inhibited obviously the Na K ATPase activity in the susceptible strain, but no effect in the resistant strain