四株糖单孢菌分离株的分类学研究

TAXONOMIC STUDY OF FOUR ISOLATES OF SACCHAROMONOSPORA

从广西地区的土样中，分离到４株细胞壁Ⅳ型，糖型Ａ，无枝菌酸的假诺卡氏菌科的放线菌菌株，编号分别为１９１、２２１、２０２、和２１２。根据４株菌的形态学特征和细胞化学特征，将其归入糖单孢菌属。与该属５个已知种的７个代表株进行的ｒＤＮＡ的ＢａｍＨＩ酶切片段长度类型分析（Ｒｉｂｏｔｙｐｉｎｇ）的结果表明：１９１为青绿色糖单孢菌（Ｓ．ｖｉｒｉｄｉｓ），２０２为青灰色糖单孢菌（Ｓ．ｃａｅｓｉａ），２２１和２１２为相同的与青绿色糖单孢菌（Ｓ．ｖｉｒｉｄｉｓ）的亲缘关系最近的种，但不同于已知的任何一个种。

Four strains, 19.1,22.1,202,and 212, were isolated from the soil samples of Guangxi province in China. Basing on their morphological and cell chemical characteristics(cell wall type IV, sugar type A, without mycolic acids), they were circled into the genus of Saccharomonospora. They were studied with 7 type strains of the 5 currently recognized species in the genus. The results of Ribotyping suggest that strain 19.1 blongs to the species of S.viridis, strain 202 is a member of S.caesia, strain 22.1 and strain 212 are the same species, which is different to the 5 currently known species. It is closely related to S.viridis. However, the further classification of these two strains at species level needs 16S rRNA sequence date.