摘要
对一株长春地区B亚型分离株(CC169)的G蛋白基因进行了序列分析,结果表明:我国呼吸道合胞病毒(RSV)分离株CC169同RSVB亚型原型株CH18537的核苷酸同源性为94%,核苷酸的有义突变率达65%。由核苷酸推导出氨基酸序列的同源性为894%,氨基酸的变异全部发生在胞外区,并主要集中在一个高度保守区的两端,胞内区和跨膜区保守不变。氨基酸的变异导致了分离株既有糖基化位点的改变,又有蛋白长度的变异。此外还初步探讨了我国RSVB亚型分离株CC169的G蛋白基因同原型株之间的变异与疫苗研制中的意义。
The nucleotide sequence of the G protein gene of respiratory syncytial virus (RSV) CC169 strain isolated from China that has been identified as subgroup B with monoclonal antibodies, was determined from cDNA that had been amplified by RT PCR and cloned into pTZ18R plasmid vector. The homology of nucleotide was 94% as compared with G protein cDNA of a RSV prototype strain (CH18537). Deduced amino acid identity of G protein was 89.4%. The amino acid changes were only in the extracellular part of the protein where there were two extensive divergent domains with a highly conserved region in between; whereas the cytoplasmic and transmembrane domains were conserved. This study demonstrates the sequence diversity of the G protein of subgroup B RSV between a Chinese isolate and the prototype strain CH18537.
出处
《微生物学报》
CAS
CSCD
北大核心
1999年第1期23-28,共6页
Acta Microbiologica Sinica
基金
卫生部科学研究基金
北京市科技新星计划