摘要
研究了离体条件下胡杨(Populus euphratica Oliver)茎段、叶片及愈伤组织的器官发生和植株再生技术。离体培养以MS为基本培养基并附加40mg/L腺嘌呤和500mg/L水解乳蛋白。离体叶片和茎段在BA为0.5mg/L和NAA为0.5mg/L的培养基上诱导产生愈伤组织,并在含0.25mg/LBA和0.5mg/LNAA的培养基上继代增殖。BA为0.5mg/L和NAA为0.1mg/L可诱导叶片和愈伤组织发生不定芽,诱导频率分别为100%和82.9%,对于茎段,BA和NAA分别为0.1mg/L和0.01mg/L时诱导不定芽频率可达83%。试管苗在大量元素减半并附加0.015mg/LNAA的MS培养基上诱导生根,生根率达86.2%。
The research of organogenesis and in vitro plantlet regeneration of Populus euphratica Oliver wascarried out using the tender shoots from mature tree as initial explants and MS medium as the basic medium.The effects of plant growth regulators (PGR) on the regeneration were compared. The results showed that theconcentation of PGR was not strictly required for the organogenesis of the excised olgans and callus, but theratio of BA to NAA was important. Calli could be induced from the excised leaves and stems cultured on themedium with 0.5 mg/L BA and 0. 5 mg/L NAA. The embryonic callus could be multiplied in dark on themedium supplemented with 0. 25 mg/L BA and 0. 5 mg/L NAA. For the adventitious bud regeneration of theleaf and callus, supplement with 0. 5 mg/L BA and 0. 1 mg/L NAA was appropriate, giving a regenerationfrequency of 82.9% and 100%, respectively. The suitable level of BA and NAA for the excised stem's was0. 1 mg/L and 0. 01 mg/L respectively, yielding a regeneration frequency of 83%. Rooting occurred on theMS medium with half strength of macronutrient and addition of 0.015 mg/L NAA, and the rooting rate couldreach up to 86. 2%. The techniques of somatic cell cloning of P. euphratica was established in vitro. Theproblems of deterioration of the subcultured shoots were also discussed.
基金
国家教委优秀青年教师基金
关键词
胡杨
器官发生
再生植株
Populus euphratica,Organogenesis, Plantlet regeneration in vitro