紫草素通过调控RANTES抑制子宫内膜异位症单核细胞募集

Shikonin inhibits the chemotaxis of monocytes in endometriosis via regulating RANTES expression

目的:解析紫草素对子宫内膜异位症(内异症)中RANTES表达及其作用的影响。方法:制备人鼠嵌合型内异症动物模型,同时体外培养单核细胞系U937细胞,用紫草素处理后,实时定量PCR法分析RANTES转录水平,ELISA法检测RANTES分泌水平,趋化实验体外评价紫草素对RANTES募集U937细胞的影响。结果:虽然紫草素促进SCID小鼠移植人子宫内膜RANTES转录(P<0.05),但仅小剂量紫草素促进其分泌RANTES(P<0.05),而中、大剂量紫草素对子宫内膜分泌RANTES无明显影响(P>0.05)。紫草素抑制U937细胞转录和分泌RANTES(P<0.05),且明显抑制RANTES趋化U937细胞(P<0.05)。结论:紫草素对单核细胞表达RANTES发挥降调作用,并且能够抑制RANTES募集单核细胞,该双重作用可能通过控制腹腔炎症而治疗内异症。

Objective：To evaluate the effect of shikonin on the expression of RANTES and chemotactic activity of monocyte in endometriosis.Methods：Established SCID endometriosis models and cultured U937 cells were treated by a series of concentration of shikonin.RANTES transcriptive expression was determined by Real-time PCR,and RANTES secretion was determined by ELISA.Furthermore,Chemotaxis assay in vitro was conducted to elucidate the effect of shikonin on chemotaxis of U937 cells by RANTES.Results：Shikonin improved the RANTES transcription of human endometrium transplanted to SCID mouse（P0.05）,whereas only low-dose shikonin promoted the RANTES secretion （P0.05）.In vitro study of U937 cells,it was showed that shikonin reduced RANTES expression at both mRNA and protein level,and inhibited obviously the chemotactic effect of rhRANTES on U937 cells（P0.05）.Conclusion：Shikonin down-regulates RANTES expression and further inhibits the migration of mononuclear cells,which might lead to alleviating inflammation of endometriosis.Shikonin may be a useful new approach to treating endometriosis.