胎儿头皮毛囊内黑素细胞HMB-45和NKI/beteb的表达

Location of melanocytes in hair follicle of fetal scalp

目的:观察黑素细胞在胎儿头皮内的定位。方法:获取胎儿头皮(胎龄7月,畸形引产,死亡2h),制备冰冻切片,分别用HMB-45和NKI/beteb抗体进行免疫荧光染色和免疫组化染色,激光共聚焦显微镜和倒置显微镜观察摄片。结果:NKI/beteb阳性细胞主要位于胎儿头皮的毛囊外根鞘隆突区,也见于毛球处、表皮基底部。HMB-45阳性细胞主要存在于毛球部、毛母质和表皮基底部,外根鞘区表达则阴性。结论:胎儿头皮毛囊内存在不同状态的黑素细胞,位于毛囊外根鞘隆突区的黑素细胞是黑素母细胞。

Objective To explore the location of melanocytes in hair follicle of fetal scalp.Methods The scalp was from a died fetus （fetal age of 7 months,induction delivery due to developmental abnormity） and prepared for frozen sections,then which respectively received immunofluorescent and immunofluochemical staining with HMB-45 and NKI/beteb antibodies.The stain results was observed and photographed by confocal microscope and the inverted microscope,respectively.Results The positive cells stained by NKI/beteb antibody were mainly located at the bulge regions in the outer root sheath （ORS） of hair follicle,some in the hair bulbs and the epidermal basilar parts.The HMB45 positive cells were present in the hair bulb,the hair matrix and the epidermal basilar parts,but not in ORS.Conclusion There are melanocytes with different status in hair follicle.Melanocyte at the bulges of ORS is melanoblast.