HPLC分析甘草中甘草酸和甘草苷含量

Analysis of Glycyrrhizic Acid and Liquiritin from Glycyrrhiza uralensis Fish. by HPLC

研究不同产地、不同生长期的野生与栽培甘草中甘草酸、甘草苷含量的变化,确定其最佳采收期。为了更好地开发利用甘草资源提供理论依据,建立了分析方法,为甘草的现代质量评价体系提供参考数据,扩大甘草药源。采用高效液相色谱测定不同产地、不同生长期野生与栽培甘草中甘草酸、甘草苷的含量。甘草酸在0.4—6μg范围内具有良好的线性关系,r=0.9999,平均回收率(n=3)为101.83%,RSD=0.74%;甘草苷在0.4—6μg范围内具有良好的线性关系,r=0.9998,平均回收率(n=3)为100.50%,RSD=0.22%。该方法确立了甘草的最佳采收期为9月中旬,种植基地的甘草可代替野生甘草使用,为甘草的药用资源和深入开发利用提供了基础依据。该方法简便、精密度高,结果准确可靠。

The authors studied the contents of Glycyrrhizic acid and liquiritin from Glycyrrhiza uralensis Fish.of wild and cultivation in different origin and different vegetation,determined the optimal picking time of Glycyrrhiza uralensis Fish.to develop and use the resources of Glycyrrhiza uralensis Fish.,and to provide the theoretical foundation.Then the authors established the methods of analysis for Glycyrrhiza uralensis Fish.,inder to provide the reference data about system of quality evaluation for Glycyrrhiza uralensis Fish.,to expande the medicinal herbs resource of Glycyrrhiza uralensis Fish..The contents of Glycyrrhizic acid and liquiritin from Glycyrrhiza uralensis Fish.of wild and cultivation in different origin and different vegetation period were determind by HPLC.The linear range was 0.4-6.0μg（r=0·9999,n=3）for Glycyrrhizic acid,the average recovery（n=3） was 101.83%,RSD=0.74%.The linear range was 0.4-6.0μg（r=0·9998,n=3）for liquiritin.The average recovery（n=3） was 100.50%,RSD=0.22%.This method established the best harvesting period of Glycyrrhiza uralensis Fish.in mid-September.It may replace Glycyrrhiza uralensis Fish.of wild with Glycyrrhiza uralensis Fish.of planting base,and develop and use better resources of Glycyrrhiza uralensis Fish.providing foundational foundation.The method was simple,accurate and reliable.