非小细胞肺癌中BRCA1、ERCC1和EGFR的表达及临床病理意义

EXPRESSION AND CLINICOPATHOLOGICAL SIGNIFICANCE OF BRCA1、ERCC1 AND EGFR IN NON-SMALL LUNG CARCINOMA

目的研究BRCA1、ERCC1和EGFR在非小细胞肺癌(NSCLC)中的表达及预后意义。方法用免疫组化ElivisionTM plus法,检测77例非小细胞肺癌和6例癌旁组织中BRCA1、ERCC1和EGFR的表达情况。结果非小细肺癌中BRCA1、ERCC1、EGFR在癌中的表达显著高于癌旁组织,且在癌中的表达具有正相关性;BRCA1、ERCC1在不同病理类型和分期中表达均无差异;BRCA1高表达组的生存期明显小于低表达组;EGFR有淋巴结转移组阳性表达率明显高于无淋巴结转移组;应用铂类药物化疗ERCC1低表达组的生存期明显明显长于高表达组。结论在NSCLC中BRCA1、ERCC1、EGFR在肺癌中的表达显著高于癌旁组织,BRCA1高表达组的生存期明显小于低表达组,EGFR高表达可能促进肿瘤的侵袭和转移,ERCC1是铂类治疗独立的预后因素,三项抗体的检测与预后和临床用药密切相关。

Objective To investigated the expressions of BRCA1、ERCC1 and EGFR in patients with non-small cell lung carcinoma（NSCLC） as well as their clinical prognostic significance.Methods The expression of BRCA1、ERCC1、EGFR in 77 cases of NSCLC and in 6 cases of adjacent normal lung tissue samples of lung carcinoma by immunohistochemistry with ElivisionTM plus method.Results The expression levels of BRCA1、ERCC1 and EGFR were significantly higher in lung cancer tissues than that in adjacent normal lung tissues.There were significant correlations between intratumoral expression of BRCA1、ERCC1 and EGFR.The expression of BRCA1 and ERCC1 had no difference between different pathological classification and clinical TNM stages.The patients with high expression of BRCA1 had significant shorter survival time than those with low expression of BRCA1.Overexpression of EGFR in NSCLCS was related to lymph node metastasis（P〈0.05）.The patients with low expression of ERCC1 had significant longer survival time than those with high expression.Conclusion The expression levels of ERCC1,BRCA1 and EGFR are significantly higher in lung cancer tissues than that of adjacent normal lung tissues.The patients with high expression of BRCA1 had significant shorter survival time than those with low expression.In NSCLC,the EGFR may play an important role in papillary thyroid cancer progression.The difference expression of ERCC1 might be a molecule marker of chemotherapy sensitivity in NSCLC patients with cisplatin-based chemotherapy.Detection of BRCA1、ERCC1、EGFR is valuable for clinical and evaluation of prognosis for non-small cell lung carcinoma.