地黄水提液对BALB/c小鼠脾细胞体外增殖作用的研究

STUDY ON PROLIFERATION OF SPLEEN CELLS IN BALB/C MICE STIMULATED BY REHMANNIA GLUTINOSA LIBOSCH WATER EXTRACT IN VITRO

目的了解地黄水提液对BALB/c小鼠脾细胞的体外增殖活性。研究三个产地、二个煎次、五个浓度的生地黄与熟地黄水提液对BALB/c小鼠脾细胞的增殖作用,以筛选出活性最强的一种。方法用三个产地的生地黄和熟地黄分别制备二个煎次、五个浓度的水煎剂,用MTT法检测对BALB/c小鼠脾细胞体外增殖作用,析因分析探讨产地、煎次、浓度等因素对增殖作用的影响。结果第一煎次的河南产熟地黄的增殖作用最强,差异有统计学意义(P<0.0005)。药物种类、产地、浓度、煎次四个因素之间有交互作用。结论三个产地的生地黄和熟地黄水提液对BALB/c小鼠都具有免疫调节作用,以河南熟地黄第一煎剂作用最强。

Objective To study the proliferation of spleen cells in BALB/c mice stimulated by rehmannia glutinosa libosch water extract in vitro.Screen out the best one of RGLE from three producing areas with two decoctions in five concentrations.Methods Dried radix rehmanniae（DRR） and radix rehmanniae glutinosa libosch（RRGL） water extract（RGLE）from three producing areas were taken and marinated respectively.Lymphocyte proliferation was measured by MTT colorimetry technique at 492 nm and the rate of proliferations were calculated to screen out the best decoction.Results Statistical analysis indicated that RRGLE was significantly better than DRRE（P〈0.0005）.The difference among three producing area was remarkably（P〈0.0005）.The proliferation was in a dose-effect dependent manner.The concentration of 10% was the best.The first decoction was notably better than the second（P〈0.0005）.There was interaction among the factors of medicine,producing area,concentration and decoction.Conclusion All of DREE and RRGLE from three producing areas could effectively regulate BALB/c mice immunity function.Henan RRGLE is the best.