通冠胶囊对大鼠颈动脉内皮损伤后血管VEGF eNOS表达和血清NO浓度的影响

Effects of Tongguan Capsule on the Expression of VEGF eNOS in Intima and Serum Level of NO in Rats after Carotid Endothelium Injury

目的:探讨通冠胶囊促进动脉损伤内皮修复的分子机制。方法:建立大鼠左颈总动脉内皮损伤模型,24只雄性SD大鼠随机等分为治疗组、对照组和模型组,治疗组给予通冠胶囊水煮液(600mg/kg)灌胃,对照组给予辛伐他汀(2mg/kg),模型组予生理盐水(10mL/kg);8周后,取材HE染色和弹力纤维染色,计算机图像分析系统检测颈动脉内皮损伤处内膜面积(intima area,IA)、中膜面积(media area,MA)和内膜/中膜面积比(IA/MA),免疫组织化学法检测VEGF和eNOS的表达,分光光度法测定血清NO水平。结果:治疗组、对照组的IA和IA/MA均小于模型组(P<0.01),治疗组、对照组血管内膜VEGF、eNOS表达和血清NO水平与均高于模型组(P<0.05或P<0.01),治疗组的血清NO水平高于对照组(P<0.05)。结论:通冠胶囊促进损伤内皮修复的分子机制可能与VEGF-AKT-eNOS-NO信号转导通路有关。

Objective：To investigate the molecular mechanism of Tongguan Capsule accelerating the recovery of arterial endothelium injury.Methods：24 male Sprague-Dawley rats which the left common carotid arteries injured were randomly divided into3 groups（n=18）：Group 1（10mg·kg^-1·d^-1 of PS was given after injury）,group 2（2mg·kg^-1·d^-1 of Simvastatin was given after injury）and group 3（600mg/kg/d of Tongguan capsule was given after injury）.After 8 week,the injured left common carotid arteries were removed for HE and elastic fibers staining and intima area（IA）,media area（MA）and IA/MA were analyzed by Computer Image Analyzer.The expression of VEGF and eNOS in injured endothelium was detected with immunohistochemistry.The serum level of NO was determined by pectrophotometry.Results：The morphological analysis demonstrated that both Tongguan Capsule and Simvastatin remarkably decreased the area of intima and IA/MA.In comparison with Group l,Tongguan Capsule up-regulated the expression of VEGF and eNOS in tunica intima after balloon injury and increased the serum level of NO.The expression of VEGF and eNOS of Group 2 were similar to Group 3.The serum level of NO in Group 3 was higher than the level of Group 2.Conclusion：The mechanism which Tongguan capsule could promote the recovery of injured endothelium probably involves the VEGF/AKT/eNOS/NO signaling pathway.