摘要
通过模拟培养试验,研究了不同Nd3+初始浓度条件下铜绿微囊藻(Microcystis aeruginosa)的生长及生理变化。结果表明,相对BG11培养基(Nd3+初始浓度为0 mg.L-1),低初始Nd3+浓度(0.01、0.05、0.1、0.5、1 mg.L-1)条件下,随着Nd3+浓度的增加,藻细胞中叶绿素a含量、可溶性蛋白含量和过氧化氢酶(CAT)活性均呈增加趋势,促进了铜绿微囊藻生长;在Nd3+初始浓度为5 mg.L-1和10mg.L-1时,藻细胞丙二醛(MDA)含量急剧增加,CAT活性下降,藻细胞清除活性氧(ROS)能力下降,抗氧化防御体系被破坏,膜脂过氧化严重,严重抑制藻细胞生长,在初始Nd3+浓度50 mg.L-1胁迫下,铜绿微囊藻无法生长。藻细胞超微结构表明,过量的Nd3+破坏了藻细胞内的类囊体结构,胞内脂质体含量增加,细胞膜变粗糙甚至变形破碎,对藻细胞造成不可逆伤害。
To investigate the effect of neodymium on Microcystis aeruginosa,the growth and physiological changes had been studied under different initial Nd3+ concentration by lab cultured experiments.The results showed that,compared with the contro(lNd3+-free),the content of chlorophyll a(Chl-a)、soluble protein and the enzymatic activity of catalase(CAT)increased when initial Nd3+ concentration changed from 0.01 mg.L-1 to 1 mg.L-1,and the growth of Microcystis aeruginosa was stimulated;However,under 5 mg.L-1 and 10 mg.L-1 initial Nd3+ con-centration,the content of malon-diadehyde(MDA)increased immediately but the enzymatic activity of catalase(CAT)reduced,which result-ed in the decline of the Microcystis aeruginosa′s ability to eliminate the active oxygen and the ruin of antioxidant defense system,and the growth of Microcystis aeruginosa slowed down.Finally,while the initial Nd3+ concentration was 50 mg.L-1,Microcystis aeruginosa couldn′t grow anymore.The ultrastructure of Microcystis aeruginosa showed that excessive Nd3+ could lead to the decrease of thylakoid and the in-crease of fat particle,and the cell membrane became rough and even broken,which caused irreversible damage to algal cells.
出处
《农业环境科学学报》
CAS
CSCD
北大核心
2010年第7期1261-1267,共7页
Journal of Agro-Environment Science
基金
四川省教育厅重点基金资助项目(2006017)
四川省学术和技术带头人培养基金资助项目
四川农业大学引进人才科技资助项目(01402000)
关键词
铜绿微囊藻
钕
生长特性
超微结构
Microcystis aeruginosa
neodymium
growth
ultrastructure