肺靶向穿心莲内酯脂质体的制备及体外释放特征

Preparation and in vitro releasing characteristics of andrographis liposomes for targeting at lung

目的：研究肺靶向穿心莲内酯脂质体的制备方法并考察其体外释药性质。方法：采用薄膜一冻融法制备穿心莲内酯脂质体；高效液相色谱法测定包封率；用透析法考察药物体外释放性质。结果：制得的脂质体平均粒径为6．325μm，包封率大于75％，稳定性好。药物体外释放符合Higuchi方程。结论：采用薄膜．冻融法可制得具有较高包封率及稳定性的穿心莲内酯脂质体，有助于提高穿心莲内酯的肺靶向性。

Objective： To study preparation and in vitro releasing characteristics of Andrographis liposomes. Method： To take the method of film-freeze-thaw for the preparation of Liposomal andrographolide; determine entrapment efficiency by HPLC; inspect in vitro releasing characteristics by dialysis. Results： The obtained liposomes average size was 6.325μm, entrapment efficiency was greater than 75%, stable. Released drugs in vitro were consistent with higuchi equation. Conclusion： To take the method of film-freeze-thaw could obtain better entrapment efficiency and stable Andrographis liposomes, help to improve lung targeting of andrographolide.