莫西沙星对气管内插管表面流感嗜血菌生物膜的影响

Effect of Moxifloxacin on Haemophilus influenzae Biofilms Formed on Intratracheal Tubes

目的观察莫西沙星对慢性阻塞性肺疾病急性发作动物模型气管内置管表面流感嗜血菌生物膜的影响。方法气管内注入流感嗜血菌建立慢性阻塞性肺疾病急性发作的动物模型,然后气管内置入硅胶管,观察莫西沙星是否对气管内置管表面流感嗜血菌生物膜的形成和膜内菌落计数产生影响。结果莫西沙星对气管内放置的硅胶管表面形成的生物膜有抑制作用,表现为用药后硅胶管表面分离的细菌数较少(P<0.05),扫描电镜也发现在莫西沙星作用下硅胶管表面无法形成成熟的生物膜,仅见散在的细菌黏附于管壁上,个别有细菌的散在团状聚集,但尚未融合呈膜状。结论莫西沙星是对气管内置管表面流感嗜血菌生物膜形成有抑制作用的抗菌药物。

OBJECTIVE To determine the effect of moxifloxacin on Haemophilus influenzae biofilms formed on indwelled intratracheally silicone tube in animal model of acute exacerbatim of chronic obstructive plelmonary disease（AECOPD） in vivo.METHODS Intratracheal instillation of porcine pancreatic elastase developed pulmonary emphysema in golden hamsters,which were infected by agar beads enclosing H.influenzae to establish animal model of AECOPD.Moxifloxacin was administered to AECOPD golden hamsters to detect the alterations of biofilms formed on the intratracheal silicone tubes by scanning electronic microscope and viable cell counting（VBC）.RESULTS Moxifloxacin exerted an inhibitory effect on biofilms formed on the surface of silicone tube indwelled intratracheally.VBC of biofilms on silicone tube was significantly reduced by moxifloxacin（P〈0.05）;and no mature biofilms were formed on the surface as a result of drug administration.Only scattered bacteria adhered on the surface without fusion as biofilm.CONCLUSION Moxifloxacin is an effective drug to antagonize H.influenzae biofilms formed on the indwelled intratracheally silicone tubes in animal model of AECOPD.