1472株酵母菌的菌株鉴定与药敏分析

Identification and Susceptibility Analysis of 1472 Strains of Yeasts

目的了解临床分离的酵母菌分布及对氟康唑与伏立康唑的敏感性。方法将医院2006-2008年临床分离的酵母菌接种于Chromagr显色培养基35℃培养48 h,无色或颜色不典型者再用API 20C鉴定;依据CLSIM-44A方案进行药敏试验,通过BIOMIC系统读取抑菌圈直径,记录数据及统计分析。结果白色假丝酵母菌最多见,占57.0%,热带假丝酵母菌占15.4%,光滑假丝酵母菌占15.4%,克柔假丝酵母菌占3.3%,丝孢酵母菌属毛孢子菌属占2.1%,其他酵母菌占6.8%;伏立康唑对多数酵母菌有效,抗菌活性优于氟康唑。结论应重视临床分离酵母菌的菌种鉴定,有必要开展酵母菌的耐药监测。

OBJECTIVE To realize the distribution and antifungal susceptibility to fluconzole and voriconazole of the yeasts isolated from clinical specimen.METHODS The clinical yeasts were inoculated in 2006-2008 on the Chromagr,and incubated at 35℃ for 48 hours.Identified the achromatic or atypical strains by API 20C Performed antifungal susceptibility in vitro according to the CLSI M-44A,measured the zones and analyzed data through BIOMIC system.RESULTS Candida albicans were the most,accouting for 57%,Candida tropicalis,Candida glabrata,Candida krusei,Trichosporon sp.and the other yeasts accouting for 15.4%,15.4%,3.3%,2.1%,6.8% respectively.Voriconazole was effective to the most yeasts and its activity was better than Fluconzole.CONCLUSION Identification of the clinical yeasts should be pay attention and surveillance of antifungal resistance is neccesary.