摘要
目的了解临床分离的酵母菌分布及对氟康唑与伏立康唑的敏感性。方法将医院2006-2008年临床分离的酵母菌接种于Chromagr显色培养基35℃培养48 h,无色或颜色不典型者再用API 20C鉴定;依据CLSIM-44A方案进行药敏试验,通过BIOMIC系统读取抑菌圈直径,记录数据及统计分析。结果白色假丝酵母菌最多见,占57.0%,热带假丝酵母菌占15.4%,光滑假丝酵母菌占15.4%,克柔假丝酵母菌占3.3%,丝孢酵母菌属毛孢子菌属占2.1%,其他酵母菌占6.8%;伏立康唑对多数酵母菌有效,抗菌活性优于氟康唑。结论应重视临床分离酵母菌的菌种鉴定,有必要开展酵母菌的耐药监测。
OBJECTIVE To realize the distribution and antifungal susceptibility to fluconzole and voriconazole of the yeasts isolated from clinical specimen.METHODS The clinical yeasts were inoculated in 2006-2008 on the Chromagr,and incubated at 35℃ for 48 hours.Identified the achromatic or atypical strains by API 20C Performed antifungal susceptibility in vitro according to the CLSI M-44A,measured the zones and analyzed data through BIOMIC system.RESULTS Candida albicans were the most,accouting for 57%,Candida tropicalis,Candida glabrata,Candida krusei,Trichosporon sp.and the other yeasts accouting for 15.4%,15.4%,3.3%,2.1%,6.8% respectively.Voriconazole was effective to the most yeasts and its activity was better than Fluconzole.CONCLUSION Identification of the clinical yeasts should be pay attention and surveillance of antifungal resistance is neccesary.
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2010年第13期1978-1979,共2页
Chinese Journal of Nosocomiology
关键词
酵母菌
鉴定
药敏试验
Yeasts
Identification
Antifungal susceptibility