新疆5个民族慢性乙型肝炎患者HBV基因型分布特点

Distribution Characteristics of HBV Genotypes from 5 Different Ethnic Nationalities in Xinjiang

目的研究新疆5个民族HBV基因型分布特征。方法应用荧光定量聚合酶链反应(PCR)和反向点杂交法检测620例样本中HBV-DNA载量和基因型,并经DNA测序对反向点杂交HBV分型结果进行验证。结果汉族、维族、哈族、蒙古族、回族C型分别为52.65%、61.96%、61.53%、73.53%、71.43%,B型分别为37.14%、14.13%、15.38%、5.88%、14.28%,D型分别为6.94%、23.37%、20.28%、20.59%、7.14%,B/C混合型占2.45%、0.54%、2.80%、0、7.14%,C/D混合型和B/D混合型仅检出1例。结论新疆地区5个民族HBV的基因型主要以C型和B型为主,其次是D型和B/C混合型。

OBJECTIVE To study the distribution characteristics of HBV genotypes in the Han,Uygur,Kazak,Mongolian and Hui nationalities in Xinjiang.METHODS Totally 620 serum samples of chronic HBV patients were selected and detected by reverse dot-blot hybridization and FQ-PCR for the distribution of genotypes and the HBV-DNA load,the positive results were investigated by DNA sequencing.RESULTS The infection rates of genotype C of the HBV in above-mentioned different ethnic groups were 52.65%,61.96%,61.53%,73.53% and 71.43%,respectively;while that of genotype B were 37.14%,14.13%,15.38%,5.88% and 14.28%;of genotype D were 6.94%,23.37%,20.28%,20.59%,0,7.14%;and of mixed genotype B/C were 2.45%,0.54%,2.80%,0 and 7.14%,respectively.Only one case was mixed genotype B/D and it was the same with mixed genotype C/D.CONCLUSIONS The genotypes of the HBV virus from the five nationalities in Xinjiang are mainly belong to genotype C and B,then the genotype D and the mixed genotype B/C.