血培养病原菌分布及耐药性分析

Pathogens Isolated from Blood Culture Specimens and Their Distribution and Drug Resistance

目的了解血培养中病原菌的分布及耐药情况,以指导临床合理用药。方法采用Bactec 9120型全自动血培养仪进行培养,MicroScan全自动微生物鉴定仪进行鉴定和药敏试验。结果 3277份血培养标本中分离出病原菌452株,阳性率13.8%;其中,革兰阳性菌占48.9%,革兰阴性菌占43.8%,真菌占7.3%;最常见的病原菌分别是凝固酶阴性葡萄球菌、大肠埃希菌、金黄色葡萄球菌,未发现耐万古霉素革兰阳性球菌,革兰阴性杆菌对亚胺培南高度敏感。结论血培养病原菌种类复杂,耐药率高,临床医师应及时监测病原菌的变化及耐药趋势,应取合理的抗菌治疗,控制医院感染。

OBJECTIVE To investigate the distribution and the drug resistance of pathogens in blood culture to conduct reasonable usage of antimicrobial agents in clinic.METHODS The blood samples were cultivated by using the Bactec 9120 system.Isolated colonies were identified and drug sensitivity was tested by MicroScan system.RESULTS A total of 452 strains of pathogenic bacteria were isolated from 3277 blood specimens,the positive rate was 13.8%.Gram-positive and Gram-negative bacilli bacteria accounted for 48.9% and 43.8%,respectively,and fungi for 7.3%.The most frequent pathogenic bacteria isolated were coagulase negative Staphylococcus,Escherichia coli and Staphylococcus aureus.No Gram-positive cocci strains were resistant to vancomycin.Gram-negative bacilli were highly susceptible to imipenem.CONCLUSIONS There are various pathogens identified from blood culture,and the drug resistance rate is high.The clinicians should monitor the changes in pathogens and trends of drug resistance in time,in order to give the patients correct antibiotic therapy and to control the cross-infection in hospital.