多粘菌素B可消除内毒素对脂质体介导基因转染的影响

ELIMINATION OF THE EFFECTS OF ENDOTOXIN ON LIPOSOME-MEDIATED GENE TRANSFECTION BY POLYMYXIN B

目的探索应用多粘菌素Ｂ（ＰＭＢ）阻止内毒素（ＬＰＳ）对转染影响。方法用活性检测法观察ＬＰＳ及ＰＭＢ对脂质体－ＤＮＡ复合物转染培养细胞后基因表达的影响。结果质粒制剂中ＬＰＳ的存在明显降低转染后基因表达水平；培养基中加用ＰＭＢ（３０μｇ／ｍｌ）可去除ＬＰＳ的这种作用。结论转染过程中ＰＭＢ的添用可清除ＬＰＳ对转染的不利影响。

Objective: To study the prevention of the effects of endotoxin (or LPS) on gene transfection by polymyxin B (PMB). Methods: Using activity assay, the effects of endotoxin and PMB on gene expression levels were identified. Results: The existence of LPS in DNA preparation reduced the gene expression remarkably during gene expression while the preparation with PMB (30μg/ml) could get rid of the effect. Conclusion: The use of LPS-binding antibiotic PMB during transfection can eliminate the harmful effects of LPS on gene expression.